The reconstitution of l-3-glycerophosphate-cytochrome c oxidoreductase from l-3-glycerophosphate dehydrogenase, ubiquinone-10 and ubiquinol—cytochrome c oxidoreductase
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چکیده
منابع مشابه
L-3-glycerophosphate dehydrogenase from pig brain mitochondria.
1. A method is described for extraction and partial purification of mitochondrial l-3-glycerophosphate dehydrogenase from pig brain. 2. By the criteria that have so far been applied, the extraction and purification procedures do not modify the activity of the enzyme towards artificial electron acceptors. 3. The amounts of acid-liberatable flavine and iron in the preparation were measured. 4. A ...
متن کاملDirect interaction between yeast NADH-ubiquinone oxidoreductase, succinate-ubiquinone oxidoreductase, and ubiquinol-cytochrome c oxidoreductase in the reduction of exogenous quinones.
The reduction of the following exogenous quinones by succinate and NADH was studied in mitochondria isolated from both wild type and ubiquinone (Q)-deficient strains of yeast: ubiquinone-0 (Q0), ubiquinone-1 (Q1), ubiquinone-2 (Q2), and its decyl analogue 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone (DB), duroquinone (DQ), menadione (MQ), vitamin K1 (2-methyl-3-phytyl-1,4-naphthoquinone), th...
متن کاملThe reaction of mitochondrial L-3-glycerophosphate dehydrogenase with various electron acceptors.
1. The kinetics of the reaction of glycerophosphate dehydrogenase with a variety of electron acceptors have been investigated. 2. In all cases the reaction mechanism appears to involve a free modified-enzyme intermediate. 3. With some electron acceptors, the maximum velocity of the reaction and the K(m) for glycerophosphate are independent of the nature of the electron acceptor, whereas in othe...
متن کاملSpecificity and locale of the L-3-glycerophosphate-flavoprotein oxidoreductase of mitochondria isolated from the flight muscle of Sarcophaga barbata thoms.
1. The oxidation of l-3-glycerophosphate by flight-muscle mitochondria isolated from the flesh fly Sarcophaga barbata has been studied. Use of substrate analogues indicates that the catalytic and effector l-3-glycerophosphate binding sites on the allosteric l-3-glycerophosphate-flavoprotein oxidoreductase differ markedly in specificity. 2. The l-3-glycerophosphate-cyanoferrate oxidoreductase sy...
متن کاملQuinol - cytochrome c Oxidoreductase from the Thermophilic Bacterium PS 3 PURIFICATION AND PROPERTIES OF A CYTOCHROME bcl
A quinol-cytochrome c oxidoreductase (cytochrome b c l complex) has been purified from plasma membranes of a thermophilic Bacillus, PS3, by ion-exchange chromatography in the presence of Triton X100. The purified enzyme shows absorption bands at 561-562 nm and 553 nm at room temperature, and 560, 551, and 547 nm at 80 K upon reduction, and gives an ESR signal similar to that of a Rieske-type ir...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1980
ISSN: 0264-6021
DOI: 10.1042/bj1920019